Glycan analysis of IgG1 type monoclonal antibodies expressed in CHO cell lines
8 Neutral standards mixture including Man5, truncated MGF and biantennary structures covering >98% of glycans on CHO mAbs.
The kit also includes three 2,3-sialylated biantennary standards and a protocol for sialylated glycans stabilization.
Also includes a protocol for antibody denaturation helping the PNGase F to deglycosylate the Fab region.
mAbsolute kits have been optimized for the MALDI-TOF based absolute glycan quantification using Bruker Autoflex and UltrafleXtreme III equipment. Validation is on-going for the application of the kits to different MALDI-TOF instruments and to other mass spectrometry techniques.