Glycan analysis of human serum IgG1 antibodies, Fc and Fab regions.
12 standards mixture including mannosides, truncated, biantennary and bisected structures. Also includes four 2,6-sialylated standards and a protocol for sialyl glycan stabilization.
The kit includes a protocol for antibody denaturation helping the PNGase F to deglycosylate the Fab region.
mAbsolute kits have been optimized for the MALDI-TOF based absolute glycan quantification using Bruker Autoflex and UltrafleXtreme III equipment. Validation is on-going for the application of the kits to different MALDI-TOF instruments and to other mass spectrometry techniques.